Кафедра біотехнології

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Відповідальний за розділ: старший викладач кафедри біотехнології БАБІЙ Віталій Олександрович. E-mail: babiy.vitaliy@npp.nau.edu.ua

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Browsing Кафедра біотехнології by Author "Bežo, Milan"

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    Changes in expression of BetV1 allergen of silver birch pollen in urbanized area of Ukraine
    (Taylor & Francis Group, 2013) Žiarovská, Jana; Labajová, Mária; Ražná, Katarína; Bežo, Milan; Śtefunová, Veronika; Shevtsova, Tetiana; Garkava, Kateryna; Brindza, Ján
    The aim of the study was to determinate the level of expression of silver birch allergen Betv1 in pollen samples from different Ukraine areas by RT-qPCR SYBR Green assay. Protocol for quantifying the expression of Betv1 allergen was developed when testing of three housekeeping genes—cyclophylin, alpha-tubulin and transcription factor CBF1. Samples from urbanized area was analysed by real-time PCR when a sample from forest growth conditions was used as a calibrator. Real-time PCR based quantifying of Bet v 1 provides a useful method for rapid and sensitive analyses of this silver birch allergen. Our results show higher expression levels in samples from central parts of urbanized area as housing estates when compared to the samples from borders of the urbanized area.
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    Chem. listy 108, 10801083(2014) Laboratorní přístroje a postupy 1080 antioxidačná aktivita peľu briez vo vzťahu k expresii betv1 alergénu
    (2014) Гаркава, Катерина Григорівна; Žiarovská, Jana; Shevtsova, Tetiana; Gažo, Ján; Brindza, Ján; Bežo, Milan
    The aim of the study was to determine the total anti-oxidant activity and expression of silver birch allergen BetV1 in pollen samples from various Ukraine areas. The obtained results show higher expression of BetV1 allergen in samples from central parts of urbanized areas (ca. 1.5 times) compared with the samples from borders of the urbanized areas (ca. 0.55 times). The total antioxidant ac-tivity of aqueous pollen extracts ranged from 80.5 % to 85.5 %, for ethanolic extracts 60.3–84.9 %. Positive corre-lation was found between antioxidant activity in ethanolic pollen extract and BetV1 allergen expresion.