Кафедра біотехнології

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Відповідальний за розділ: старший викладач кафедри біотехнології БАБІЙ Віталій Олександрович. E-mail: babiy.vitaliy@npp.nau.edu.ua

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Browsing Кафедра біотехнології by Subject "57.017.3"

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    ВПЛИВ ЗНИЖЕНОЇ ТЕМПЕРАТУРИ НА СИНТЕЗ ФЛАВОНОЇДІВ У КУЛЬТУРАХ «БОРОДАТИХ» КОРЕНІВ ARTEMISIA VULGARIS L. ТА ARTEMISIA ANNUA L.
    (Українське товариство генетиків і селекціонерів ім. М.І. Вавілова, 2018) Ястремська, Лариса Сергіївна; Матвєєва, Н.А.; Гаврилюк, О.А.
    The aim of the work was to investigate the effect of short-term cold stress (+10°С) on the growth, flavonoid synthesis and antioxidant activity in Artemisia vulgaris L. and A. annua L. “hairy“ root cultures. Methods. Transgenic roots were cultivated during the first 1, 2 and 5 days at +10°C on Murashige and Skoog basal medium with twice reduced macrosalt content. The total flavonoids content in Rutin equivalent was determined using alcohol extract reac-tion with alumunium chloride. Antioxidant activity was studied using the DPPH method. Results. Short-term cold stress resulted in a reduction of mass increment by 12–76 %. The total flavonoid content in «hairy» roots ranged from 32.0±3.13 to 187.0±21.04 mg RE/g dry weight. Decrease of temperature has led to decrease of the flavonoids content in No. 1, No. 2 A. vulgaris root lines and No. 5 A. annua line by 18–33 %. The reaction of No. 3 A. vulgaris and No. 4 A. annua root lines was expressed in stimulation flavonoid synthesis by 62 % and 56.5 %. Cultivation of «hairy» roots under short-term cold stress has led to decrease of the antioxidant activity in all roots lines by 4–40 %. Conclusions. Сold stress had negative effect the “hairy“ roots growth, stimulated flavonoids accumulation only in two “hairy” root lines and reduced the level of antioxidant activity.