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Title: Validation of HMG CoA Reductase as Internal Control for Hazelnut Pollen Allergens Expression Analysis
Authors: Garkava, Katerina
Žiarovská, Jana
Nikolaieva, Natalia
Brindza, Ján
Гаркава, Катерина Григорівна
Keywords: Corylus avelana
HMG CoA reductase
Pollen allergens
Issue Date: 13-Feb-2015
Citation: Žiarovská, J., Nikolajeva, N., Garkava, K., & Brindza, J. (2015). Validation of HMG CoA reductase as internal control for hazelnut pollen allergens expression analysis. Austin. J. Genet. Genom. Res, 2, 1-4
Abstract: In the study, 3-hydroxy-3-methylglutaryl coenzyme A reductase gene was validated for its application in hazelnut pollen allergens CorA and profillin expression analysis. HMG CoA reductase was used as internal control in expression analysis by one-step real-time PCR approach. Pollen sample from rural area from Sumy region (Ukraine) was used as the calibrator and the sample from Kamenets-Podolskiy (cement plant area; Ukraine) was chosen for the expession analysis. Using one-step real time PCR approach, following values of expression level were obtained in this study: 0,63 x higher for CorA and 51,6 higher x for profilin hazelnut pollen ellergen. The results and parameters of expression profile data are comparable to the two-step real-time PCR, that were previously reported for Corylus avelana, L. pollen allergens and confirmed HMG CoA reductase as suitable internal control for the CorA and profillin expression analysis.
ISSN: 2471-030X
Appears in Collections:Наукові праці співробітників кафедри біотехнології

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