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dc.contributor.authorGarkava, Katerina-
dc.contributor.authorŽiarovská, Jana-
dc.contributor.authorNikolaieva, Natalia-
dc.contributor.authorBrindza, Ján-
dc.contributor.authorГаркава, Катерина Григорівна-
dc.identifier.citationŽiarovská, J., Nikolajeva, N., Garkava, K., & Brindza, J. (2015). Validation of HMG CoA reductase as internal control for hazelnut pollen allergens expression analysis. Austin. J. Genet. Genom. Res, 2, 1-4uk_UA
dc.description.abstractIn the study, 3-hydroxy-3-methylglutaryl coenzyme A reductase gene was validated for its application in hazelnut pollen allergens CorA and profillin expression analysis. HMG CoA reductase was used as internal control in expression analysis by one-step real-time PCR approach. Pollen sample from rural area from Sumy region (Ukraine) was used as the calibrator and the sample from Kamenets-Podolskiy (cement plant area; Ukraine) was chosen for the expession analysis. Using one-step real time PCR approach, following values of expression level were obtained in this study: 0,63 x higher for CorA and 51,6 higher x for profilin hazelnut pollen ellergen. The results and parameters of expression profile data are comparable to the two-step real-time PCR, that were previously reported for Corylus avelana, L. pollen allergens and confirmed HMG CoA reductase as suitable internal control for the CorA and profillin expression analysis.uk_UA
dc.subjectCorylus avelanauk_UA
dc.subjectHMG CoA reductaseuk_UA
dc.subjectPollen allergensuk_UA
dc.titleValidation of HMG CoA Reductase as Internal Control for Hazelnut Pollen Allergens Expression Analysisuk_UA
Appears in Collections:Наукові праці співробітників кафедри біотехнології

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