Browsing by Author "Poliukh, Kateryna Igorivna"

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Technology of obtaining proteases from mycelial fungi
(National fviation university, 2021-06) Полюх, Катерина Ігорівна; Poliukh, Kateryna Igorivna
Proteases represent a large and diverse group of hydrolytic enzymes that are classified by their site of action, enzyme active site structure, and specific reaction mechanisms The use of proteases in different industries has been prevalent for many decades and a number of microbial sources exist for the efficient production of this enzyme. Their vast diversity, specific range of action and property of being active over a very wide range of temperature and pH have attracted the attention of biotechnologists worldwide. Although they are widely distributed in nature, fungi are the preferred source of these enzymes in fermentation bioprocesses because of their fast growth rate and also because they can be genetically engineered to generate new enzymes with desirable abilities or simply for enzyme overproduction. The search for new microorganisms that can be used for protease production is a continuous process. Proteases have various applications in major areas of food processing, beverage production, animal nutrition, leather, paper and pulp, textiles, detergents, etc. and with the advent of new frontiers in biotechnology, the spectrum of protease applications has expanded into many new fields such as clinical, medicinal and analytical chemistry. Some species of filamentous fungi, such as Aspergillus, Penicillium and Paecylomices have been identified as great producers of extracellular protease in submerged fermentation. Further studies on the optimized conditions will still be performed and the protease production will be conducted in bioreactors.