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Title: Changes in expression of BetV1 allergen of silver birch pollen in urbanized area of Ukraine
Authors: Žiarovská, Jana
Labajová, Mária
Ražná, Katarína
Bežo, Milan
Śtefunová, Veronika
Shevtsova, Tetiana
Garkava, Kateryna
Brindza, Ján
Keywords: Quantitation real-time PCR
Bet v 1 allergen
Betula pendula Roth.
Issue Date: 2013
Publisher: Taylor & Francis Group
Citation: Changes in expression of BetV1 allergen of silver birch pollen in urbanized area of Ukraine / J. Žiarovská, M. Labajová, K. Ražná [et al.] // Journal of Environmental Science and Health, Part A. – 2013. – № 48. – Р. 1479 –1484.
Series/Report no.: 48;
Abstract: The aim of the study was to determinate the level of expression of silver birch allergen Betv1 in pollen samples from different Ukraine areas by RT-qPCR SYBR Green assay. Protocol for quantifying the expression of Betv1 allergen was developed when testing of three housekeeping genes—cyclophylin, alpha-tubulin and transcription factor CBF1. Samples from urbanized area was analysed by real-time PCR when a sample from forest growth conditions was used as a calibrator. Real-time PCR based quantifying of Bet v 1 provides a useful method for rapid and sensitive analyses of this silver birch allergen. Our results show higher expression levels in samples from central parts of urbanized area as housing estates when compared to the samples from borders of the urbanized area.
ISSN: 1093-4529 (Print)
1532-4117 (Online)
Appears in Collections:Наукові праці співробітників кафедри біотехнології

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