SEROLOGICAL AND BIOLOGICAL ACTIVITY OF LIPOPOLYSACCHARIDE FROM Escherichia coli L-19
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Date
2015
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Abstract
The lipopolysaccharide from Escherichia coli L-19 was isolated, chemically identified and its nontoxicity
but pyrogenicity were shown. The investigations of lipopolysaccharide influence on T- and
B-lymphocytes indicate that it may be used as a mitogen in blasttransformation reactions since it is only
in insignificant degree less active in comparison with the commercial one. It was shown that in reactions
of Ouchterlony double immunodiffusion in agar LPS from E. coli L-19 exerts an activity of antigen in
homological system. There were not established serological cross reactions between antiserum to heated
E. coli L-19 cells and LPS from another of E. coli strain М-17 аnd also the representatives of
Enterobacteriaceae species such as Budvicia aquatica, Rahnella aquatilis and Pragia fontium. These
results indicate the absence of common antigenic determinants between the tested strains.
Description
Keywords
Escherichia coli lipopolysaccharide, toxicity, pyrogenicity, mitogenic activity
Citation
Garkavaya K.G. Serological and biological activity of lipopolysaccharide from Escherichia coli l-19 / K.G. Garkavaya, L. D. Varbanets, E. L. Zdorovenko, O. S. Brovarskaya // Biotechnologia acta. -2015.- v. 8.- No 1. - p.32-38.