Changes in expression of BetV1 allergen of silver birch pollen in urbanized area of Ukraine

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dc.contributor.authorŽiarovská, Jana
dc.contributor.authorLabajová, Mária
dc.contributor.authorRažná, Katarína
dc.contributor.authorBežo, Milan
dc.contributor.authorŚtefunová, Veronika
dc.contributor.authorShevtsova, Tetiana
dc.contributor.authorGarkava, Kateryna
dc.contributor.authorBrindza, Ján
dc.date.accessioned2014-10-01T19:09:14Z
dc.date.available2014-10-01T19:09:14Z
dc.date.issued2013
dc.description.abstractThe aim of the study was to determinate the level of expression of silver birch allergen Betv1 in pollen samples from different Ukraine areas by RT-qPCR SYBR Green assay. Protocol for quantifying the expression of Betv1 allergen was developed when testing of three housekeeping genes—cyclophylin, alpha-tubulin and transcription factor CBF1. Samples from urbanized area was analysed by real-time PCR when a sample from forest growth conditions was used as a calibrator. Real-time PCR based quantifying of Bet v 1 provides a useful method for rapid and sensitive analyses of this silver birch allergen. Our results show higher expression levels in samples from central parts of urbanized area as housing estates when compared to the samples from borders of the urbanized area.uk_UA
dc.identifier.citationChanges in expression of BetV1 allergen of silver birch pollen in urbanized area of Ukraine / J. Žiarovská, M. Labajová, K. Ražná [et al.] // Journal of Environmental Science and Health, Part A. – 2013. – № 48. – Р. 1479 –1484.uk_UA
dc.identifier.issn1093-4529 (Print)
dc.identifier.issn1532-4117 (Online)
dc.identifier.urihttp://er.nau.edu.ua/handle/NAU/10122
dc.language.isoenuk_UA
dc.publisherTaylor & Francis Groupuk_UA
dc.relation.ispartofseries48;
dc.subjectQuantitation real-time PCRuk_UA
dc.subjectBet v 1 allergenuk_UA
dc.subjectBetula pendula Roth.uk_UA
dc.titleChanges in expression of BetV1 allergen of silver birch pollen in urbanized area of Ukraineuk_UA
dc.typeArticleuk_UA

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